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Important safety considerations
• All points of connection in liquid chromatographic systems
are potential sources of leaks. Users should be aware of
the toxicity or flammability of their mobile phases.
• Because of the small particle size, dry column packings
are respirable. Columns should only be opened in a well
ventilated area.
• Please adhere to operating pressure limits noted for
each column (see table). Exceeding these limits will
compromise chromatographic performance and could
be unsafe.
Other operating tips
• While generally not harmful to the column, reverse flow
should be avoided except to attempt removal of clogged
frit (see Column care).
• Always use high purity reagents and chromatography
grade solvent to prepare your mobile phase. Degas and
filter all mobile phase prior to use.
• Disassembling a column will degrade column
performance.
• New columns contain a mixture of organic solvents and
water. See your QC Performance Report for the solvent
composition in your column. Initially, care should be taken
not to pass any mobile phase through the column that
may cause a precipitate to form.
• Agilent reversed-phase columns are compatible with water
and all common organic solvents.
• The use of a guard column is recommended to protect
your column and increase its lifetime.
• Columns should not be maintained at elevated pH or
elevated temperature when not in use.
• Avoid use of this column outside of recommended
pH ranges for column phase (see next page).
Expect reduced lifetime when operating outside the
recommended pH and temperature ranges.
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