TROUBLESHOOTING
If the incubator does not seem to be working properly, check the following items before calling a service
engineer:
Problem
The incubator does not operate
at all.
An alarm is activated.
The chamber temperature does
not match the set value.
The chamber humidity does not
rise.
The CO
gas density does not
2
match the set value.
A large quantity of gas is being
consumed.
Cannot culture properly and
the gas density in the
chamber may be the cause.
CO
gas is not being injected.
2
Items to be checked and countermeasures
Is the incubator plugged in?
Is there a power outage, or has a circuit breaker interrupted the power?
Is the removable power supply cord connected to the port on the lower right side of the
cabinet?
[When starting operation]
Does the chamber temperature match the set value?
Does the CO
gas density in the chamber match the set value?
2
(1) Is the secondary pressure for the gas regulator at the specified value of
0.03 MPa(G)–0.1 MPa(G) (0.3 kgf/cm
(2) Is the gas tube properly connected?
[During operation]
Has the high-limit alarm temperature been set at least 1 C higher than the
temperature set for the chamber?
Has the temperature setting been changed? Has the outer door been left open for a
long time? Has a low-temperature object been placed in the chamber? If any of these
is the case, the alarm will clear automatically after a short time.
Has the gas tube come loose, or is there a gas leak?
Has the CO
gas density setting been changed?
2
Is the gas cylinder empty? Check the primary pressure of the gas cylinder once a
week (A primary pressure reading of 3.8 MPa(G) (38 kgf/cm
is a sign that there is little gas remaining. Replace the cylinder soon).
Is the incubator operating beside an appliance that generates electromagnetic waves?
Is the ambient temperature at least 5 C lower than the temperature set for the
chamber?
Is the outer door closed with the inner door left open?
Is the incubator operating beside an appliance that generates electromagnetic waves?
Is there enough water in the humidifying pan (be sure to use sterile distilled water)?
Is the secondary pressure for the gas regulator at the specified value of
0.03 MPa(G)–0.1 MPa(G) (0.3 kgf/cm
Is the gas tube blocked?
Is the duct securely attached? (See page 84)
Is the fan attached properly? (See page 84)
Is the incubator operating beside an appliance that generates electromagnetic waves?
Are the outer and inner doors being frequently opened and closed?
Check whether gas is leaking from connectors due to deterioration of the gas tube, or
whether there may be any pinhole leaks. The gas tube is a consumable part, and it is
recommended to replace the tube once a year.
Is the packing seal for the inner door defective?
Is the access hole open?
Is the air environment around the incubator normal? Is there a source of polluted gas
in the vicinity?
CO
control for the incubator follows the ON-OFF method. CO
2
injected as the gas density in the chamber approaches the set value. Injections may
be stopped for periods of approximately 15 seconds, but that is not an error.
The gas is not injected until the temperature of the CO
stable, approximately 1 hour after turning ON the power switch or recovering from
power failure.
2
2
(G)–1 kgf/cm
(G), 4.4 psi(G)–14.5 psi(G))?
2
(G), 551 psi(G)) or lower
2
2
(G)–1 kgf/cm
(G), 4.4 psi(G)–14.5 psi(G))?
sensor becomes sufficiently
2
gas is intermittently
2
83