Items Required But Not Provided - helena BioSciences SAS-3 Immunofix Instrucciones De Uso

ITEMS REQUIRED BUT NOT PROVIDED

Cat. No. 310200 Sample Applicator Blades (1 x 10)
Cat. No. 310300 Sample Applicator Blades (5 x 10)
Cat. No. 210100 Disposable sample cups (100)
Cat. No. 3100 REP Prep
Saline solution (0.85% NaCl)
The following items are not required for standard serum IFE but may be required for further
investigation and Urine IFE investigation:
Cat. No. 320100 Antiserum to Human Urine Total Protein (5ml)
Cat. No. 320200 Antiserum to Human Urine Micro Proteins (5ml)
Cat. No. 320300 Antiserum to Human Urine Macro Proteins (5ml)
Cat. No. 320400 Antiserum to Human GAM Proteins (5ml)
Cat. No. 320700 Antiserum to Human Free & Bound Kappa Light Chain (5ml)
Cat. No. 320800 Antiserum to Human Free & Bound Lambda Light Chain (5ml)
Cat. No. 320500 Antiserum to Human Free Kappa Light Chain (5ml)
Cat. No. 320600 Antiserum to Human Free Lambda Light Chain (5ml)
Cat. No. 320900 Antiserum to Human Urine Pentavalent/Albumin (5ml)
Cat. No. 321000 Antiserum to Human Urine Pentavalent (5ml)
Cat. No. 9249 Antiserum to Human IgD
Cat. No. 9250 Antiserum to Human IgE
Cat. No. 9400 IFE Control Kit
SAMPLE COLLECTION AND PREPARATION
Freshly collected serum is the specimen of choice. Samples can be stored at 15...30°C for up to 4 days,
2...6°C for up to 2 weeks or 6 months at -20°C
Urine samples should be applied neat. Serum samples should be diluted according to the following
table using Sample Diluent:
Monoclonal Concentration
25-50g/L
10-25g/L
3-10g/L
<3g/L
STEP-BY-STEP PROCEDURE
1. Program the following parameters into the instrument:
Step
Electrophoresis
Load Sample
Apply Sample
Electrophoresis
Apply antisera
Insert Combs
Blotter D
Dry
* Use Location 2 for SPIFE 3000 and SAS-3.
6
.
SP Lane
Time (mm:ss)
00:30
00:30
06:30
10:00
02:00
05:00
08:00
1+2
1+2
1+2
1+2
Temperature (°C)
21
21
21
21
21
40
54
3
SAS-3 IMMUNOFIX
G, A, M, κ κ , , λ λ
1+19
1+9
1+4
Neat
Voltage Other
Speed 1
Speed 1*
650
English