Installing stage inserts
1.
Install the holder in the mechanical stage. (Fig. 19)
2. Multi well plates can be directly inserted in the mechanical
stage. (Fig. 20)
Aperture diaphragm
The Numerical Aperture (N.A.) value of the aperture diaphragm
affects the image contrast. Increasing or reducing this value one
can vary resolution, contrast and depth of focus of the image.
With low contrast specimens move the Aperture Diaphragm
①
lever (AS)
to set the numerical aperture to about 70%-80%
of the objective's N.A. (Fig. 21)
If necessary, remove one eyepiece and, looking into empty
sleeve, adjust the aperture diaphragm ring in order to obtain an
image like the one in Fig. 22.
vwr.com I Instruction manual INVERTED MICROSCOPE, VISISCOPE
APERTURE
DIAPHRAGM
FIELD OF VIEW
IT417 PH
®
F F ig. 19
ig. 19
F ig. 20
F
ig. 20
①
F ig. 21
F
ig. 21
70-80%
30-20%
F ig. 22
F
ig. 22
18