VWR avantor VISISCOPE IT417 PH Manual De Instrucciones página 20

Use of the microscope in phase contrast
Installing the phase contrast slider
1. Insert the slider into the lower slot of the illumination sys-
tem, printed face up. (Fig. 24)
2. Pull the slider into the desired position, until it arrives to the
click stop.
3. When in phase contrast observation, keep the aperture di-
aphragm adjustment lever
Phase contrast slider
• The phase ring is pre-centered when the microscope leaves
the factory. It should therefore need no further adjustment.
Should a re-centering is needed, it can be performed via
the two side bolts (see Chapter "Centering the phase ring").
The 4x/10x position ② must be used with 4x and 10x phase
•
contrast objectives, the 20x/40x position ③ with the 20x
and 40x and the SL position ④ is used for brightfield. (Fig.
25)
SLIDER
MEANING
POSITION
SL empty hole
4x/10x phase ring 4x/10x
20x/40x phase ring 20x/40x
Centering the phase ring
• Usually this operation is not needed. If necessary, please
proceed with the following steps:
1. Place a specimen on the stage and focus it.
2. Take out the eyepiece from the eyepiece tube without the
diopter adjustment, and replace it with the centering tele-
scope (CT). (Fig. 26)
3. Check that the phase ring and the objective match, and
that both are steadily set on a click stop.
vwr.com I Instruction manual INVERTED MICROSCOPE, VISISCOPE
①
on the "O" (open) position.
APPLICATION
brightfield
observation
phase contrast
observation with 4x
and 10x objectives
phase contrast
observation with 20x
and 40x objectives
①
②
IT417 PH
®
F ig. 24 F ig. 24
③ ④
F ig. 25 F ig. 25
F ig. 26 F ig. 26
20