Assembly; Operating Instructions - Biohit Biotrate Manual Del Operador

Assembly

The Biotrate and Biotrate Pro are packed with the dispense nozzle attached and the inlet feed tube removed. The 300mm
length of PTFE inlet tube provided should be trimmed to fit your particular container. The bottom end should be cut diago-
nally at a length that is close to the bottom of the container when the Biotrate or Biotrate Pro is assembled to the contain-
er. Longer lengths of inlet tube are available on request.
Four adaptors are available as accessories to suit containers with a 33mm, 38mm, 40mm or 45mm screw neck. The thread-
ed platform base of both Biotrates has a 30mm screw thread for use with containers of this size.
The assembled Biotrates are screwed to the reservoir using gentle hand torque applied to the threaded platform base (10)
only. Removal should also be by means of hand torque applied to the threaded platform base only.
Do not operate the titrate wheels (1) until the unit is safely and fully mounted onto the reservoir!

Operating instructions

Four momentary action push buttons
1 'On' - switches the unit on and displays the last reading (15).
2 'Reset' - reverts display to 00.00 (18).
3 'CAL' - user custom calibration - pen point access (17).
4 'Mode' - toggles between aspirate or titrate on the LCD display (16).
Priming
Position an empty container under the right angle dispense nozzle (12) open anti-drip valve (11) and move the piston to the
fully down postion. The dispense nozzle must point away from the user at all times. Prime the unit by a few gentle up and
down piston strokes, taking the piston right down and lifting about an inch or so. Repeat until a steady, bubble free flow
is obtained from the dispense nozzle. Priming can be completed without switching the unit on. After priming, the piston
should be left in the fully down position. When not in use turn anti-drip valve to the closed position.
Titrating
To titrate, first switch the instrument on by pressing the 'ON' button. Press 'MODE' until the LCD arrow points upwards.
Press 'RESET' to zero the unit. Open anti-drip valve and gently turn the titrate wheels (1) 'up' so filling the borosilicate glass
barrel (8). The amount of fluid taken in should be greater than the volume required for titration and will be displayed on
the LCD screen.
Note: Too fast a filling action might lead to air bubbles forming under the piston.
After each filling, compensate for backlash by turning the hand wheels slightly in the discharge direction until 1-2 drops are
dispensed into an empty container.
To start titration, press the 'MODE' button (16) so that the arrow points down. Press the 'RESET' button (18) to zero the
display. Place a receiving vessel under the dispense nozzle (12) and gently turn the titrate wheels (1) 'down' so dispensing
liquid. The dispensed volume will be shown on the display. Do not drive the piston to the bottom of its stroke, but stop a
few ml short of it. If necessary, the syringe may be refilled at any time during the titration, without affecting the dispensed
volume shown on the LCD using freeze count feature.
Freeze Count Feature
Biotrate has a unique 'Freeze Count' feature enabling the user to freeze the displayed value. This function allows backlash
compensation after replenishing the barrel during titration, without affecting the value displayed. Simply press and hold 'ON'
button, aspirate to refill barrel. Release 'ON' button to continue titration. It also allows the user to take a sample of the dis-
pensed liquid during titration, just press 'ON' button to 'Freeze Count'.
Auto off
The Biotrate will automatically switch off when it is idle for a period of more than three minutes. The instrument is switched
on by pressing the 'ON' switch. When the instrument is switched on, the previous value is displayed. For subsequent titra-
tion, press the 'RESET' button (18) to zero the display.
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Biotrate Operator Instructions