Cálculos
Tabla 1Cálculos del software "Biotest"
Nombre del
Ensayo
DNA/Proteina
Factor de dilución (D f ) = vol diluyente + volumen muestra A
(260, 280)
Concentración DNA = [(A
Concentración proteinas
Relación = A
DNA (260, 230)
Factor de dilución
Concentración DNA
Concentración proteinas
Relación = A
DNA (260, 280)
Factor de dilución
con barrido
Concentración DNA
Concentración proteinas
Relación= A
DNA (260, 230)
Factor de dilución
con barrido
Concentración DNA
Concentración proteinas
Relación= A
Cálculo(s)
volumen muestra
– A
)f
1
ref
1
= [(A
– A
2
- A
1
ref
A
- A
2
ref
(D
) =
vol diluyente + volumen muestra
f
volumen muestra
= [(A
– A
)f
1
ref
1
= [(A
– A
2
- A
1
ref
A
- A
2
ref
(D
) =
vol diluyente + volumen muestra
f
volumen muestra
= [(A
– A
)f
1
ref
1
= [(A
– A
2
- A
1
ref
A
- A
2
ref
(D
) = vol
diluyente +
f
volumen muestra
volumen muestra
= [(A
– A
)f
1
ref
1
= [(A
– A
2
- A
1
ref
A
- A
2
ref
C-1
Apéndice C Cálculos
Parámetros
A
A ref = 320nm
– (A
– A
)f
]D f
2
ref
2
f
1
)f
– (A
– A
)f
] D
f
ref
3
1
ref
4
f
2
f 3 = 1552
f
4
vol. dil. = 0
vol. mstra = 1
A
A
A
– (A
– A
)f
] D
2
ref
2
f
f
1
)f
– (A
– A
)f
] D
f
ref
3
1
ref
4
f
2
f
3
f
4
vol. dil = 0
vol nstra= 1
LO de inicio
LO final
– (A
– A
)f
] D
2
ref
2
f
A
)f
– (A
– A
)f
] D
A
ref
3
1
ref
4
f
A
f
1
f
2
f
3
f
4
vol. dil. = 0
vol mstra. = 0
estándar
A
A
A
– (A
– A
)f
] ] D
2
ref
2
f
f
1
)f
– (A
– A
)f
] D
f
ref
3
1
ref
4
f
2
f
3
f
4
dil.vol. = 0
Manual del operador BioMate 3
[Default]
Resultados
mostrados
µg/mL
=
1
= 280nm
2
(opcional)
= 62.9
= 36,0
= 757.3
µg/mL
= 260nm
1
= 230nm
2
= 320nm
ref
(
)
opcional
= 49.1
= 3.48
= 183
= 75.8
µg/mL
= 225nm
= 325nm
= 260nm
1
= 280nm
2
= 320nm
ref
(opcional)
= 62.9
= 36.0
= 1552
= 757.3
µg/mL
= 260nm
1
= 230nm
2
= 320nm
ref
(opcional)
= 49.1
= 3.48
= 183
= 75.8