Maintenance And Cleaning - Nahita MICROTOMO DE MANO 501 Manual De Usuario

1. Fit the glass stage on the microtome wide flat platform. If the glass stage does not fit,
turn it until it does. If the glass stage is loose on the platform, you can hold it with silicone
to avoid it moving.
2. Open the microtome as wide as it can go by rotating the screw on the side. You can see
the clamp open or close by looking inside the cylindric central hole.
3. Put the sample you want to cut into the hole and tighten it securely with the clamp by
rotating the screw. Note: The microtome can hold specimens up to 1 cm of diameter and
sometimes, a carrot or other material can be used to support the specimen if it is
necesary.
4. Take the razor and slice off any excess of specimen by cutting against the flat platform
until the sample is flush with the glass stage. Attention!: For your own safety always
move the razor blade away from you.
5. Turn the knurled micrometric knob at the bottom of the microtome so the sample rises to
just above the top of the microtome. The knob is graduated in 10 m (0.01mm) steps and
advances 500 m (0.50mm) for each complete turn. The thinest section which can be
obtained with the hand microtome is 10 m-thin.
6. Take the razor blade and cut again, just like before, to obtain a thin section of the
specimen.
7. Apply a drop of water to the center of a clean microscope slide and put the thin slice on
top of the water. Cover the section with a cover glass and it is ready to see under the
microscope. Note: Most of the times the cut should be stained to observe the different
structures under the microscope.
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5. MAINTENANCE AND CLEANING

To get the best results and a higher duration of this equipment it is essential to follow the processes of use.
Note: All the processes of use mentioned below will not have any value unless you keep a continued and
careful maintenance.

Please follow the processes of use of this manual.

This manual should be available for all users of this equipment.

Always use original components and supplies. Other devices can be similar but they can damage the
equipment.

Never try to repair the hand microtome by yourself

In the event of breakdown please contact your distributor to overhaul through Nahita Technical
Assistance Department.
Revisión 4 de Abril 2017 Manual de instrucciones ZFP001 Pág. 9 de 6