Running A Program - Amersham Pharmacia Biotech EPS 601 Manual Del Usuario

EPS 601 - User Manual
Back to start position
The program is now back to the start position in the SET mode with the program number flashing. It is
stage during programming in SET mode by pressing
possible to go back to this position at any
EXIT or STOP. Note that the program is saved automatically when EXIT is pressed.
Disabling the current check
See Section 5.4 if you want to use this feature.

5.3 Running a program

Connect the leads from the electrophoresis unit (red to red, and black or blue to blue). Red is
positive and black or blue negative. Up to two electrophoresis units can run at the same voltage
at one time. Please remember to double the maximum current and power conditions if two
units are to be run. Voltage will be the same regardless of how many units are run. The current
should also be doubled if two gels are run on the same unit.
Choosing a program
Press SET and select the program you wish to run by pressing / until the value after the "P" is
(Omit this step if you have just programmed a method as
correct. Confirm with ENTER.
described in section 5.2.)
Running a program is described schematically in Fig. 4.
Running
Press RUN to start the electrophoresis. The display will show current values for voltage, current, power
and elapsed time or volthours. You can switch between showing the elapsed time or volthours by / .
The limiting parameter will attain the programmed limit. The unit for that parameter is underlined. A
light emitting diode shows when voltage is applied (HV on). If no current is displayed, please check the
electrical connections to the electrophoresis equipment.
Pausing
You can interrupt the electrophoresis for sample loading and/or changing parameter values by pressing
PAUSE CONTINUE. Voltage and current will no longer besupplied, the HV on LED goes off, and you
may safely load your samples. The display shows the status of the run when PAUSE CONTINUE was
pressed. Switch between time and integrated voltage by pressing / . When sample loading is complete,
press PAUSE CONTINUE again or RUN and the run will continue from where it was interrupted. When
in the PAUSE mode you can also press SET to make changes in the program. This mode is called the P-
SET mode. When the P-SET mode is entered you can make changes as described under programming.
The P-SET mode is the same as the SET mode apart from restrictions in setting the breakpoint.
Naturally, it is not possible to enter a time or volthour that is already passed. Press EXIT or PAUSE
CONTINUE to go back from P-SET to PAUSE. Press RUN or PAUSE CONTINUE to proceed with the
electrophoresis.
View programmed values
It is also possible to view the programmed values during a run by pressing SET. Note that no values can
be changed here. The display returns automatically to show RUN values after 5s. Alternatively use EXIT
or RUN.
Stopping the run and viewing end parameter values
When the programmed time or volthour is attained, the program will enter the End mode. It is also
possible to break the run manually by pressing STOP. In both cases, the voltage, current and power will
go to zero as indicated by the HV on LED switching off. The end parameter values are displayed. Switch
between elapsed time and integrated voltage by / . An alarm will sound if selected in the program.
You can stop the alarm by pressing STOP.
A run cannot be continued after pressing STOP.
8