Running A Program - Amersham Pharmacia Biotech EPS 3501 XL Manual Del Usuario

EPS 3501 XL - User Manual
Select phase number by / and move in the program with SET ENTER to the value to be changed.
Change with / and confirm with SET ENTER. Press EXIT.
Inserting and deleting a phase
Inserting and deleting a phase is described schematically in Fig. 6.
Note that you must first enter a phase before it can be inserted or deleted:
1. If needed, change program number by entering the start position in SET mode with SET ENTER or
EXIT, changing the number with / and confirming by SET ENTER.
2. Bypass the mode question with SET ENTER.
3. Change to the desired phase number by / and confirm with SET ENTER.
Choose to delete the phase shown on the display or insert a new phase with this phase number by
pressing / and confirm with SET ENTER.
If DELETE is chosen, the selected phase will be deleted and the program will move back to the position
with the phase number blinking. Note that by deleting a phase, the numbers of the following phases will
decrease by 1.
If INSERT is chosen, the program will enter the same position and the new phase can be programmed as
a new phase (see section 5.2 and Fig. 3, points 4–8). After entering the breakpoint, the question "Last
Phase?" will be bypassed and the starting point for the next phase will be entered. Note that by inserting
a new phase the number for the old phase with that number and the numbers for the following phases
will increase by 1.
Adding a phase after the last phase is done by entering the last phase as above, moving to the question
"Last Phase?" and answering NO. The program will jump to the start position for programming a phase
with the phase number blinking. Proceed with programming as described for a new program.
It is not possible to insert or delete a phase for a running program.
Editing a running program
See section 5.4
Copying and clearing a program
See section 5.5.

5.4 Running a program

Connect the leads from the electrophoresis unit (red to red, and black or blue to blue). Red is positive
and black or blue negative. Up to two electrophoresis units can run at the same voltage at one time.
Please remember to double the maximum current and power conditions if two units are to be run.
Voltage will be the same regardless of the number of units. The current should also be doubled if two
gels are run on the same unit.
Running a program is described schematically in Fig. 7.
Choosing a program
Press SET ENTER and select the program you wish to run by pressing / until the value is correct.
(Omit this step if you have just programmed or edited a method as described in sections 5.2 and 5.3.)
Running
Press RUN to start the electrophoresis. Information about the status of the start current check will be
displayed for a few seconds. The display will then show current values for voltage, current and power
and one of elapsed time, volthours or milliamperehours. You can switch between showing the
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