VWR VisiScope BL524 P Manual De Instrucciones página 7

Adjust interpupillary distance
Hold the right and left parts of the observation head by both hands and adjust the interpupillary distance by
turning the two parts until one circle of light can be seen. After finishing the interpupillary adjustment, turn the
dioptric adjustment ring on the left eye tube to the "0" position at the graduation line on the eyetube.
Focus and diopter adjustment
Loosen the focus-stop knob, rotate the coarse focusing knob to bring the slide into focus with an objective
with a low magnification, and then lock the focus-stop knob. Adjust the fine focusing knob to get the image
sharp and clear while observing with your right eye, then turn the left dioptric adjustment ring to a sharp and
clear image also with the other eye. Turn the tension-adjust-knob to get a suitable tension for the focus sy-
stem. When the image is in focus, turn the nosepiece to choose the objective you need.
Condenser adjustment
Turn the condenser adjustment knob to move the condenser up or down for a clear and uniform sample illu-
mination. The two condenser centring screws can be used to centre the condenser.
Set the numerical aperture
Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus controlling ima-
ge contrast and resolution.
Field diaphragm
Adjust the field diaphragm to align the system and to control stray light.
Additional filters
The chromatic correction filter (blue) and the monochromator filter (green) can be inserted for definition of
specimen parts. The use of the green filter is recommended for phase contrast observations.
Using the polarizer (optional)
The polarizing set consists of two filters; the analyser and the polarizer. The analyser is pla ced in the analyser
holder and the polarizer on top of the illuminator.
Video capturing (optional)
Two kinds of observation heads, binocular (standard or ergonomic) or trinocular, are available for the this
series. Trinocular version has a dedicated photo-port, while binocular version can receive a camera in either
eyepiece tube, via a photo/video adaptor. Before taking a picture or filming video, if necessary, obscure with
a dark cloth both the camera viewfinder and the eyepieces and pull out the light path selector lever. Please
refer to the adaptor and camera manuals for further details
Using the phase contrast set (optional)
The phase contrast set has 4 objectives for phase contrast and one condenser, with 4
rings of phase, and one position for bright field. To use the phase contrast, remove the
abbe condenser of the microscope and replace it with the phase contrast condenser.
If, during use, the phase contrast diaphragm rings (anuli) are dislodged, they can be rea-
ligned rotating the alignment screws placed on two sides of each ring, using the enclosed
tools. In particular:
Select an objective corresponding to the ring to be realigned, and focus on a slide under
bright field illumination.
The selector of the condenser has 5selectable positions:
0 → To use when it is necessary to be used the instrument in bright field
10 → To use with 10x phase contrast objective
20 → To use with 20x phase contrast objective
40 → To use with 40x phase contrast objective
100 → To use with 100x phase contrast objective
Insert the phase ring to be realigned.
Remove an eyepiece, and replace it with the centring telescope.
7