Recalibration - Axygen AXYPET Manual De Instrucciones

vapours, it is recommended to disassemble the shaft
and rinse the piston and seal with distilled water after
finishing the pipetting operation.

8 - RECALIBRATION

AXYPETs Pipettes are calibrated by gravimetrically,
using AXYGEN tips and distilled water, at a temperature
of 20±1°C, according to EN ISO 8655 standard.
If during pipette operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in section 1, the pipette recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been met during
error determination:
• the ambient temperature, and the temperature of the
pipette, tips and water was identical
• the density of the liquid used is close to that of distilled
water
• a balance with appropriate sensitivity has been used.
Volume checked [μl]
0.1 - 10
10 - 100
> 100
• mg/μl conversion factor has been taken into account
• the requirements shown in sections 3 and 7 have been
met
If the above conditions are satisfied and the accuracy
error for selected volume given in section 1 exceeds the
permissible value, the pipette recalibration procedure
should be carried out.
The recalibration can be performed within one full turn of
the key to the right or to the left only.
Recalibration conditions:
Ambient temperature, the temperature of the pipette, tips
and liquid should be within the range 20-25°C and
stabilised during weighing within ±0.5°C
• Measurements should be conducted using distilled
water
• Balance sensitivity should be suitable for the volume to
be measured
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Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
Recalibration procedure:
• Set the dose volume depending on the pipette volume
according to the following table:
Range
Preset
of the pipette
Model volume
volumes
[μl]
[μl]
AP-2 0.1 - 2 0.2
AP-10 0.5 - 10 0.5
AP-20 2 - 20 2
5
AP-50 5 - 50
AP-100 10 - 100 10
AP-200 20 - 200 20
AP-250 50 - 250 50
AP-1000 100 - 1000 100
AP-5000 1,000 - 5,000 1,000
AP-10ML 1,000 - 10,000 1,000
• Perform 5 aspirations, weigh each one and calculate
the average value of the aspirations
• Calculate average aspirated volume in μl multiplying
the average aspiration amount [mg] by the distilled
water density coefficient [μl/mg], which depends on
temperature and pressure according to the following
table:
Temperature [°C]
20
21
22
23
24
25
If the average aspirated volume exceeds the permissible
value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A),
Warning: The pipetting pushbutton consists of 2 parts:
the lower volume-setting section (Fig. 1A2) and the
upperbutton (Fig. 1A1). After removal of the pushbutton,
both parts will separate.
• Holding the volume setting knob to protect it against
rotation, insert the calibration key into the cuts of the
calibration screw, (Fig. 4B),
ENGLISH
Volume change
Permissible Δ Δ V for full turn
volumes of the calibration key
[μl] [μl]
(24 increments)
0.176 - 0.224 0.06
0.48 - 0.52 0.33
1.94 - 2.06 0.63
4.875 - 5.125 2.50
9.84 - 10.16 2.50
19.76 - 20.24 6.30
49.5 - 50.5 6.30
98.4 - 101.6 25.00
994 - 1,006 125.00
975 - 1,025 250.00
Pressure [kPa]
95.0 101.3 105.0
1.0028 1.0029 1.0029
1.0030 1.0031 1.0031
1.0032 1.0033 1.0033
1.0034 1.0035 1.0036
1.0037 1.0038 1.0038
1.0039 1.0040 1.0040
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