Beckman Coulter Biomek FX-ADMETox Manual Del Usuario página 7

Test Compounds
Ketoprofen
Verapamil
Antipyrine
Metoprolol
Carbamazepine
Ranitidine
Propanolol
c. Add 80 µl of each test compound solution into each well of the StockPlate.
DMSO
Ketoprofen
Verapamil
Antipyrine
Metoprolol
Carbamazepine
Rantidine
Propranolol
NOTE Extra test compound can be frozen for future use.
5. Populate the worksurface with appropriate labwares.
3 96-well UV plates
r
Biomek P200 tip boxes
r
PAMPA Sandwich Plate (pre-loaded)
r
SUPPORT Plate
r
StockPlate
r
LipidPlate
r
ASB solution Low Profile Reservoir
r
System Buffer High Profile 12 Trough Reservoir
r
Deep well plate for diluted test compounds
r
6. Enter test compound information into Excel template file.
a. Launch Microsoft Excel application.
b. Select File | Open.
c. Browse to open PAMPA Template.xlt file located in the PAMPA Evolution command
Software directory.
d. Enter each compound in the same well location that it was loaded in the StockPlate.
e. Physiochemical constants are optional.
f. Do not leave blank spaces in the Excel template sheet.
Biomek FX/PAMPA Evolution96 Quick Start and Trouble Shooting Guides
PN A29703AB
Using the PAMPA EVOLUTION96 Software and Biomek FX Workstation
MW Weight (mg)
254.3 2.54
491.1 4.91
188.2 9.40
684.8 6.85
236.3 2.36
350.9 3.51
295.8 2.96
Quick Start Guide
DMSO (ml) Concentration (mM)
1
1
1
1
1
1
1
1
10
10
50
10
10
10
10
7