Beckman Coulter Biomek FX-ADMETox Manual Del Usuario página 8

Quick Start Guide
Using the PAMPA EVOLUTION96 Software and Biomek FX Workstation
g. Rename the Excel file and save in DATA sub-folder in the PAMPA Evolution
command Software.
h. Exit the Excel application.
7. Run PAMPA assay.
a. Click the Import from Excel button on the toolbar.
b. The Input Compounds dialog box appears, click the Browse button to import test
compound Excel template file.
c. Click the OK button for the Warning dialog box for correct StockPlate type.
d. Verify the loaded compound names and physiochemical constants, click the OK
button to continue.
e. Open the Instrument-Settings, select the Use pre-measured blank.
f. Browse to select DefaultBlankPlate.spc.
g. Uncheck Tell me about low-UV wells...
h. Click the OK button and exit the Instrument Settings.
i. Click the General Information button, fill in the appropriate experimental
conditions, click the OK button.
j. Click the Start Assay button.
k. Select the Uninterrupted Assay Flow and Use pre-measured blank check boxes.
l. Verify that Double-Sink Protocol is selected, and incubation time is set to "0.5 hr".
m. Select Properties and verify the System Solution Volume = 600 µL and Stirring be
ABL = 40 µM.
n. Select Multi pH Assay and select pH Map.
o. Verify the updated layout of System Solution at different pH.
p. Click the OK button and Start the assay to proceed.
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Biomek FX/PAMPA Evolution96 Quick Start and Trouble Shooting Guides
PN A29703AB