Terragene Bionova MiniBio Manual De Usuario página 7

Instructions for use
ble to select an incubation program for each of the po-
sitions depending on the final reading characteristics of
the indicators to be used.
Check that the time setting is appropriate for the BIs to be
incubated in each position
To change the time program, press the
correspondent position as many times as necessary to
choose the right setting.
5- Wait for the temperature of the machine to be stable.
Once is stable, the indicator of the desired temperature
will remain steady.
Do not place any Biological Indicator in read posi-
tions until the incubator does not confirm tempe-
rature stability.
The incubator will keep running the incubation program
during all reading process. The system does not allow the
incubation program to be modified once the reading is
started, except it has been completed.
6- Reading can be initiated when the temperature blue
light remains stable indicating that incubation tempera-
ture was met. Then an automatic testing of the status of
the incubation positions of the biological indicators will
be performed. The indicating lights of each position will
be turned on to show the result: green light will show a
satisfactory result while red light will indicate error.
When the device detects an error in any position, it will
print a ticketindicating date, time and positions with
error. To guarantee the reliability of the results, these
positions will be deactivated avoiding their use. To make
this clear, red light in that position will remain blinking in-
definitely to indicate that the position has been disabled.
For the correct performance of the automatic
check, it should be done with the protective cover
closed.
Read BI´s instructions for use. Before placing the
vials in the incubator, press the top to seal the
tube. Crush the internal ampoule (using the am-
poule crusher within the box of BIs or the one lo-
cated within the incubation area of the incubator)
and make sure that the liquid has completely wet
the spore carrier placed at the bottom of the biolo-
gical indicator plastic tube.
Do not remove or change placement of BI once the
reading process has begun. If this occurs, results
may be invalidated.
Use a non-sterilized biological indicator as a posi-
tive control every time a processed indicator is in-
cubated. For more information refer to the Positive
Control section of this user guide.
7-Place the rapid BI in a reading position, close the pro-
button of the
tective cover and wait for the result. Reading will start au-
tomatically. Immediately after placing the BI in a position,
Bionova® MiniBio auto-reader will emit a sound alarm
indicating that reading has successfully began and status
position light will turn on and will blink until the result is
informed.
NOTE: If the incubator does not run automatically, it mi-
ght indicate that the incubator temperature is not steady
yet (check the temperature indicating light to remain ste-
ady) or that the incubation position is disabled due to an
error in the initial autotest.
8- When a positive result is detected, the red light and the
audible alarm will turn on. This will indicate that the steri-
lization process to which the BI was subject, has failed. 30
seconds after removing the IB from the incubation posi-
tion, the alarm and the red light will turn off automatically
and the position will be available to start a new reading.
The negative BIs will be informed with a green light in the
corresponding position, this will indicate that the sterili-
zation process to which it was subject has been success-
ful. The green light will remain on for up to 30 seconds
after the BI is withdrawn from the reading position.
The incubator will inform results within 30 min. (60 ºC),1
h. (60 ºC), 2 hs. (60 °C), 3 hs. (60 ºC) or 4 hs. (37 ºC), accor-
ding to the program selected. These periods of incuba-
tion are the time-limits used by the equipment to inform
the fluorescence final readout.
9- Every time a positive result is detected, it will be infor-
med through the printing of the corresponding ticket
and with an audible alarm. The alarm can be cancelled by
pressing the button
10- To register the tickets, take the paper and press up for
the paper to be cut with the serrated edge of the printer.
NOTE: If the printer runs out of paper, printer's blue light
will start blinking (see Replacement of the paper roll in the
printer). The incubator will save the last 3 results, for re-prin-
ting, follow the Reprinting of the last results instructions.
Bionova® MiniBio Page 7
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