Calibration - Star Lab ErgoOne Manual De Instrucciones

6. Calibration

During manufacture, ErgoOne
are aligned for transferring aqueous
solutions and calibrated according to
EN ISO 8655. We therefore also recom-
mend that you regularly check your
pipettes at defined intervals of, for ex-
ample, 3 or 12 months.
Generally, realignment is not necessary
with normal use. However, if your pi-
pettes still do not achieve the specified
limiting values (see section 5 Technical
Data), check the following points:
– Is the pipette seal broken?
Is it dripping? (see section 3
Troubleshooting)
– Is the density of the liquid you are
using different from that of water?
– Are there any temperature differ-
ences between the pipette, liquid
and air?
– Was the liquid pipetted slowly and
evenly?
If the check is unsuccessful, calibrate
and realign your pipette. This can be
carried out according to the following
instructions. Alternatively, STARLAB
offers a professional Pipette Calibration
Service with a fast turnaround.
Note that each alignment means a zero
point shifting. The amount by which the
pipette setting is changed is constant
across the entire measurement range.
If for example 1000µl is realigned by
10µl (1%) for a 100 –1000µl pipette,
then for 100µl, the pipette must also
be corrected by 10µl = 10%.
Calibration & alignment
pipettes
®
Calibration is a gravimetric analysis for
which you require an analytical bal-
ance adjusted to the pipetting volume,
a weighing vessel and distilled water
(room temperature).
General information
– Only use TipOne
attach these securely.
– Same temperature for pipette, tip
and water
– Each tip must be pre-wetted with
distilled water at least three times.
– Always pipette evenly and slowly
and hold the pipette upright.
– Immerse no more than 3 mm of
the pipette tip into the liquid when
taking a sample.
– Position the pipette at an angle of
30 – 45° to the inner wall of the tube
when dispensing liquid.
– For multi-channel pipettes, all
channels must be checked
separately.
pipette tips and
®
ErgoOne · User Manual ·
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