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Idiomas disponibles
Idiomas disponibles
CARE AND MAINTENANCE
Units are best cleaned using warm water and a mild detergent. Water at temperatures above 60ºC can
cause damage to the unit and components.
The tank should be thoroughly rinsed with warm water or distilled water to prevent buildup of salts, but care
should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised.
Air drying is preferably before use.
The units should only be cleaned with the following:
RNase Decontamination
This can be performed using the following protocol:
Clean the units with a mild detergent as described above.
Wash with 3% hydrogen peroxide (H2O2) for 10 minutes.
Rinse with 0.1% DEPC-(diethyl pyrocarbonate) treated distilled water,
Caution: DEPC is a suspected carcinogen. Always take the necessary precautions when using.
TM
RNaseZAP
(Ambion) can also be used. Please consult the instructions for use with acrylic gel tanks.
OPERATION METHOD
1. Add a small amount of transfer buffer (39 mmol/L glycine, 48 mmol/L Tris, 0.037% SDS, 20% methanol)
in a shallow tray, place the spongy cushion and filter paper soak in the buffer.
2. Cut the filter paper to the same size with the gel, and put the filter paper on the spongy cushion, put the
gel on the filter paper, and then put the cellulose nitrate membrane and filter paper on the gel, finally
cover with a spongy.
3. Carefully move the filter paper, spongy cushion, membrane and the gel to the transfer splint and fasten
the bayonet, insert the transfer splint to the core of the tank, add the buffer until the transfer splint
immerse in the buffer.
Note: 1. Please pay attention of the direction of positive and negative.
2. Before the experiment, please cool the electrophoresis buffer in the refrigerator.
4. Carefully place the lid on the tank and connect to a power supply, start the transfer electrophoretic.
Note: In order to ensure a good result, please keep the temperature at 4ºC.
5. After the experiment, take out the transfer splint, remove the cellulose nitrate membrane, according to
the experimental purpose to proceed the next step detection.
Manual de Instrucciones-Instruction Manual
Versión-Version 1, 02/2022
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