Aspirating And Dispensing Liquids - bioSan Assist Serie Manual De Instrucciones

The single channel pipettes
Counter
Model
Readings
1
AP2 2
5
0
AP10
7
5
1
AP20 2
5
0
AP100 7
5
1
AP200 2
5
0
AP1000 7
5
1
AP5000
2
5
0
AP10000 7
5
The multichannel pipettes
Counter
Model
Readings
0
AP8-10
3
AP12-10
5
0
AP8-50
6
AP12-50
5
0
AP8-200
8
AP12-200
5
2
AP8-300
5
AP12-300
0
To attain the maximum accuracy, set volume must be ap-
proached from a higher value.
If the desired value is lower than the previous value, simply
adjust the value down to 1/3 turn above the required setting.
10
Set Volume Basic Degree
1.25 µl 0.002 µl
7.5 µl 0.02 µl
12.5 µl 0.02 µl
75 µl 0.2 µl
125 µl 0.2 µl
0.75 ml 2 µl
1.25 ml 10 µl
7.5 ml 20 µl
Set Volume Basic Degree
3.5 µl 0.02 µl
6.5 µl 0.10 µl
85.0 µl 0.20 µl
250.0 µl 1.0 µl
If the desired value is higher than the previous value, adjust
the value to 1/3 turn above the required value. The setting
should then be performed in the following manner:
Slowly turn the pipetting pushbutton knob or the black adjust-
ment knob to the required volume.
If the knob is accidentally turned too far, below the required
value, the process must be repeated.
3 – ASPIRATING AND DISPENSING LIQUIDS
The single channel pipettes
Place a tip on the shaft of the pipette. See Section 6 for the
appropriate tip. Press the tip on firmly using a slight twisting
motion. This will ensure an airtight seal.
The multichannel pipettes
In order to put tips on the multichannel shafts hold the pipette
vertically and press it against the tips in the rack box, until the
shafts retract about 1.5 mm into the multichannel module.
The suspension system of the shafts ensures even and tight
sealing of tips. The rocking movement does not have to be
performed to seal the tips tightly.
Important: Never aspirate liquids into the Assist pipette
without a tip attached.
Aspiration
Press the pushbutton to the first positive stop, (Fig. 2A).
Holding the pipette vertically, immerse the tip into the sample
liquid. The depth to which the tip is immersed in the sample
liquid depends on the model.
Model
AP2
AP10
AP20, AP50, AP100
AP200, AP250, AP1000
AP5000
AP10000
Release the pushbutton slowly and smoothly to aspirate the
sample, (Fig. 2B). Wait one second and then withdraw the
tip from the liquid. If the pipette tip is not immersed to the
recommended depth or if the pipetting pushbutton is rapidly
released, air may enter the disposable tip.
ENGLISH
Immersion depth [mm]
≤ 1
≤ 1
2 - 3
2 - 3
3 - 6
5 - 7
11