Hoefer SQ33 Manual Del Usuario página 16

3.2 Gel Pouring
1
Remove any greasy fingerprints or water on the edges
of the glass plates by wiping with acetone. Lay the
plain glass plate on a flat surface and arrange the
spacers flush with the edges of the plate. Carefully lay
the notched glass plate on top and clamp the plates
together. The gel sandwich is now ready for pouring.
Refer to the illustration in Figure 1, page 6.
2
Fill a 60 ml syringe with gel mix being careful not to
aerate the solution.
3
Hold the syringe as vertical as possible and using
a steady continuous motion expel the gel mix along
the notched area moving the syringe nozzle repeat-
edly from one side of the notch to the other. The gel
mix should form a continuous pool along the top of
the gel space and by capillary action it will move
down between the plates. Do not overfill the notched
area — the pool should be approximately half the
height of the glass ears. Alternatively do not let the
pool run dry or air bubbles will be introduced between
the glass plates. The boundary of the gel should
migrate as a straight line. The gel plates can be
tapped slightly behind the moving gel boundary, using
your fingers, to prevent any bubble formation. Do not
use any hard or metal objects.
4
When the gel boundary reaches the bottom of the
glass plates, remove the surplus gel from the notched
area. Failure to do so will result in the gel mix drip-
ping from the bottom end. Insert the required comb.
5
If you are using a comb with square wells insert the
teeth without trapping bubbles. If you are using a
sharks tooth comb insert the flat face of the comb at
a slight angle to prevent bubbles from trapped. Add a
few drops of gel mix if necessary. Carefully straighten
the comb so that it is parallel to the top of the gel
plate and reaches 3 – 5 mm below the notched area.
•
p5