Unit Assembly - Hoefer SQ33 Manual Del Usuario

6
The gel polymerization should occur within 10
minutes but allow the gel to polymerize further for
at least 90 minutes. If it is to polymerize overnight,
enclose wet tissues under a cling film seal to prevent
the ends of the gel from drying out.
If you are using a square toothed comb do not remove
it until it is submerged below the running buffer.
However, if you use a Shark's tooth comb it should be
removed before the addition of buffer. Insert it teeth
first into the gel slot so that the teeth just come into
contact with the gel and then add the running buffer.

3.3 Unit Assembly

1
Place the lower buffer chamber into the main unit.
Push it back until it stops. This ensures that the
electrode separation and hence field strength are
consistent from one run to another.
2
Remove any clamps and the bottom tape, if used.
3
Clamp the gel plate sandwich into position on the
apparatus. Do not over tighten the gel plate clamping
screws as this will lead to the glass plates break-
ing and also making the insertion and removal of
combs difficult.
4
The gel plate assembly will rest against the aluminum
back plate. This has been set during manufacture to be
parallel to the main frame. Do not tighten or adjust the
aluminum plate securing screws. The aluminum plate
is cushion mounted to provide good contact with the
glass plate.
5
Make sure that the upper buffer tank drainage tube
is closed.
6
Attach the electrical connectors to the buffer chambers.
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