Hoefer SQ33 Manual Del Usuario página 19

IMPORTANT! Do not fill over
the maximum fill lines.
•
p8
3.4 Run Conditions and Buffer Volumes
1
Into the upper buffer chamber pour 800 ml
(approximately).
2
Into the bottom buffer chamber pour 800 ml
(approximately).
3
Ensure that there is no buffer leakage.
4
If desired pre-run the gel until the glass plates are
warm. Use the settings described below.
5
Prior to loading your samples flush out the wells with
running buffer to clear them of urea.
6
Heat the sequencing samples at 95 °C for 3 minutes,
place on ice and centrifuge 12,000 × g for 3 minutes.
Return to ice.
7
Load 1– 5 μl of sample using a suitable loading tip. If
possible avoid taking the sample from the bottom of
the tube — particulate materials may cause streaking
or smearing. Try to minimize sample dispersion, load
the sample directly onto the bottom of the well and
keep it as a thin layer.
8
Replace the safety lid ensuring it is positioned fully
down over the electrical connectors.
9
Connect and run the gel at the desired power setting.
The leads and electrical connectors are tested to
1,500 Volts and users are advised not to exceed
this voltage.