Remote Control by External Contact Closure
and Autosampler Control
The 212-LC System can be controlled from an autosampler or an injector
valve, or any external device that will provide a brief contact closure to a
terminal on the rear panel of the 212-LC pump. The six pin connector
terminal includes 3 pins labeled "START", "STOP", and "COMMON"
(Figure 2). When the 212-LC System is under autosampler control all
timing and injection functions are controlled by the autosampler. If the
autosampler requires two common outputs from Pump A to activate
Start/Stop switch closures, use a "jumper" (short piece of wire) to
connect the common terminals at the autosampler. Start/Stop functions
require a momentary pulse and should normally be left open. For
example, using the ProStar 410 AutoSampler, the inject marker wires
(black and white) in the P1 cable should be connected to the Start/Stop
contacts at the back of the 212-LC Pump A.
After a method file has been created, it is run as outlined above by
pressing Activate, Enter, then Run. The selected method file will begin
at step T0 conditions and maintain these conditions for 60 minutes
before "timing out" and automatically stopping the pump. This same "time
out" feature is active between multiple method runs, so the user must
start the original method within 60 minutes or the pump will stop. During
this time the timer (T0) will display the time in 0.10 minute increments.
The 212-LC system is now in the "Idle" condition and is ready to run the
method file. The pump returns to "Idle" after running each method file.
The "Idle" condition allows the user to equilibrate the chromatography
system and the autosampler time to carry out control functions. From the
"Idle" condition, the method file can be run by pressing Run a second
time, or by using an external device (autosampler) to provide a contact
closure (short "Start/ Common") as described above. Once the method
run has been initiated, the method will start at T0. If an autosampler is
being used to run a method multiple times, a single contact closure when
the 212-LC system is in the "Idle" condition will start the method again. If
the method Link function has been used, the system will not go into the
"Idle" condition until the Link function has been completed.
It is generally accepted as good chromatography practice to edit the
method file so the starting and ending conditions are the same.
Otherwise the method will automatically switch to the T0 conditions at
the end of the method file.
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