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Unpacking and Installation
The 212-LC System consists of two high pressure HPLC pumps.
Pump A serves as the master control pump and will also be referred to
as the pump which delivers solvent A. To distinguish the A and B pumps,
the Serial Number for the A pump is labeled as AXXXX, whereas the B
pump is labeled as BXXXX. Pump B serves as a slave pump and will
also be referred to as the pump which delivers solvent B. Unless it is
specifically noted in the text, the same general installation, repair, and
troubleshooting procedures apply to both pumps.
Important: Cut and remove the two large tie-wraps from the underside
of each pump chassis before running the pump. Cut the tie wraps and
pull them out of the chassis vents and discard. Do not cut the small white
cable tie which is also exposed as it secures the serial cables within the
chassis.
1. After unpacking the instrument, compare the contents with the
packing slip to verify that the shipment is complete. Inspect all items
and report any damage immediately to the shipping carrier, your
local distributor or Varian. If any items are damaged, save the
shipping container which may be required for claims purposes.
2. Place pumps on a level bench top with sufficient space for detectors
and other auxiliary equipment. In a gradient system, the pumps are
stacked on top of each other with Pump A on top. The pumps
should be located away from direct sunlight and from areas subject
to extreme temperature fluctuations such as air vents.
3. Install the solvent inlet line assembly and solvent filter to the pump
inlet check valve (see Figure 1). Always use a solvent filter to
protect the check valves and system hydraulics from contaminants.
Solvent bottles should be placed at approximately the same level as
the pump or higher. In special circumstances the solvent reservoir
can be located below the pump inlet (maximum 36 in.) without a
loss of pump performance.
Note: To maintain flow accuracy, it is essential to degas the solvent. The
most efficient method of degassing is the use of an inline degasser.
Alternatively, solvents can be sonicated under vacuum for approximately
15 minutes or continuously sparged with helium.
The pump is shipped with 2-propanol in the pump head. Make sure the
new solvent is miscible with 2-propanol or flush with an intermediate
solvent to ensure solvent compatibility.
4. Install the waste line in the purge valve body (1/4-28 finger-tight
fitting), with the outlet placed in a suitable solvent waste container
(see Figure 1).
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