Use Of The Microscope (B-383Fl); Use Of The Microscope (B-383Ld1 / Ld2); Use Of The Shutter - Optica B-380 Serie Manual De Instrucciones

12.4 Use of the microscope (B-383FL)

1. Turn on the power supply for the mercury bulb
and wait 5 minutes for the arc to stabilize.
Move the filter selector ① to one of the 2 available
2.
positions until the click stop. (Fig. 49).
3. The microscope has a 3-position filter holder.
The leftmost position allocates the B filter, the
central position is empty for transmitted light
observation and the rightmost position allocates
the G filter.

12.5 Use of the microscope (B-383LD1 / LD2)

1. Turn on the fluorescence LED, by switching on
"II" the main switch placed on the back side of
the frame. (Fig. 50)
Move the filter slider ① in one of the 2 available
2.
positions until the click stop. (Fig. 49).
3. LD1and LD2 models have a 3-position filter
holder. The LD1 model the slider allocates only
a B filter, while the LD2 model allocates a B and
a G filter.
FILTER EXCITATION
CUBE FILTER
B 475/30 nm
G 530/40 nm

12.6 Use of the shutter

• The microscope is equipped with a shutter
① located on the right side of the fluorescent
illuminator. (Fig. 51)
1. Close the shutter by interrupting the observation
for a limited time and not subjecting the sample
to unnecessary lighting in the period in which it
is not observed.
(Switching off and switching on frequently the
HBO lamp considerably reduces its duration).
• This precaution is not necessary in the case
of the LD1 and LD2 models: the LED can be
switched on and off without any problem.
DICHROIC EMISSION
MIRROR FILTER
505 nm 515LP nm
570 nm 590LP nm
• FITC: fluorescent antibodies
• Achridine orange: DNA, RNA
• Auramine
• Rhodamine, TRITC: fluorescent antibodies
• Propidium iodide: DNA, RNA
• RFP
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APPLICATIONS
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Fig. 49
Fig. 50
Fig. 51