Directions For Use - Sartorius Stedim Biotech Sartolab-P20 Instrucciones Para El Uso

6. Directions for Use

6.1. The Sartolab P20 and Sartolab P20 Plus
are pressure filtration units.
The liquid to be filtered can be supplied
via a pressure tank or a peristaltic pump.
Especially for this range of applications,
we offer a self-regulating diaphragm pump
with electronic speed control and a set of
connecting hoses for the Sartolab P20 units
(18059). Please read the operating instruc-
tions provided with the pump.
6.2. After you have set up the pressure
source and filtrate receptacle, remove
a Sartolab P20 unit from the box and make
sure that the packaging is not damaged.
6.3. Make sure that the inlet side of the
pressure filtration unit is located below the
seam for opening the package (marked with
a "Y"). The inlet side can be identified by the
eight vent openings arranged around the
inner circle (see Figure 1). The sides of the
unit are also labeled "IN" = inlet and "OUT"
= outlet. If you have ordered Sartolab
P20/Sartolab P20 Plus with a filling bell,
it is on the outlet side.
Once you have properly positioned the unit,
tear open the packaging at the designated
seam (see Figure 2).
6.4. Connect the hose from your pressure
source to the inlet side of the Sartolab P20/
Sartolab P20 Plus (luer lock connector or
stepped hose nipple) without taking the
sterile side out of the packaging.
Check that the hose is connected tightly
enough to withstand the pressure applied.
6.5. Without touching the sterile side,
remove the filtration unit from the packag-
ing and mount the unit above the filtrate
receptacle, for instance, using a stand
(see Figure 3).
6.6. Remove the lid from the filling bell
before beginning filtration.
6.7. Start filtration by slowly increasing the
applied pressure. The entrapped air escapes
through the PTFE membrane by automatic
venting. As soon as the cellulose acetate
membrane has completely wetted out, you
can increase the inlet pressure up to 3 bar.
6.8. After the filtration run has been
completed, you can check the integrity of
the filter by connecting a pressure source to
the unit. Adjust the inlet pressure to a con-
stant 3.0 bar (300 kPa or 44 psi) and hold
the Sartolab filtration unit under water
in a beaker.
If the filter is intact, no air bubbles should
escape from the outlet (downstream) side.
Air bubbles that escape through the
automatic vents on the upper part do not
have any effect on the integrity of the
surfactant-free cellulose acetate membrane.
6.9. After completing filtration, discard
the filtration unit. If applicable, follow the
current regulations for radioactive or
infectious material.
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