Coded Microscopes - Zeiss Axio Observer Serie Manual Breve

Axio Observer
12 HOW TO GET TO THE FIRST PICTURE
12.1

Coded microscopes

Setting transmitted-light brightfield according to KÖHLER
GENERAL WORKING ADVICE
Before starting to work with the microscope, first read the sections 3 and 8.
− The microscope has been started up correctly.
− The microscope has been switched on.
• Select the objective with the lowest magnification (e.g. objective 10x - yellow ring) on the nosepiece
(Fig. 5/13); ensure that it clicks into position correctly. Set factor 1x on the selector wheel (Fig. 5/16) of
the Optovar turret.
• Open the luminous-field diaphragm completely by turning the luminous-field diaphragm control
(Fig. 5/7) on the carrier for transmitted-light illumination to the left.
• Open the aperture diaphragm completely by rotating the selector wheel on the condenser forwards as
far as it will go.
• Turn the condenser turret adjustment ring (Fig. 5/3) to move the condenser turret to the H position for
brightfield (if H is not available, to the DIC position).
• Turn the adjustment ring to move the reflector turret (if available) to a position which contains no filter
combination. Ensure that it clicks into position correctly.
• If necessary remove the analyzer slider from the slot (Fig. 5/12) or move to the open position. Ensure
that it clicks into position correctly.
• Turn the sideport selector wheel (Fig. 5/2) to the 100 % vis (visual) position.
• Set the beam splitting ratio to 100 % vis (Fig. 5/20) on the binocular (photo)tube. Remove the
Bertrand lens from the optical path (where used). To do this, move the rotary / sliding button
(Fig. 5/21) to the 100 % vis position.
• Swivel the 3-position filter changer (Fig. 5/5) out of the optical path.
• Place a high-contrast specimen on the microscope stage (Fig. 5/10).
• Match the eyepiece distance (interpupillary distance) to the user's individual interpupillary distance: For
this purpose, pull apart or push together the binocular component (Fig. 5/22) of the tube.
• Set the ametropia correction zero point using the adjustment ring (Fig. 5/24) of the eyepieces
(Fig. 5/23): without eyepiece reticles:
with eyepiece reticles:
• To correct for ametropia, bring the selected detail of the specimen into optimum focus using the
relevant eyepiece adjustment ring.
• Use the coarse / fine focus drive (Fig. 5/17) to focus on the selected detail of the specimen. If no light is
visible in the eyepieces, check that light is being emitted from the housing of the halogen illuminator. If
no light is being emitted, switch on the halogen illuminator by pressing the TL button (Fig. 5/18).
• Set the light intensity to a comfortable level by using the illumination control wheel (Fig. 5/19).
09/2017
HOW TO GET TO THE FIRST PICTURE
set to the white point,
set to the red point.
431004-6044-008
ZEISS
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