ZEISS
Fig. 4
Diaphragm settings in transmitted
light brightfield acc. to KÖHLER
• To set the aperture diaphragm, remove one eyepiece from the eyepiece tube and adjust the aperture
diaphragm to approximately 2/3 the diameter of the exit pupil of the eyepiece (
The settings required for optimum contrast depend on the specimen.
• Reinsert the eyepiece and where necessary refocus on the specimen using the fine focusing drive.
• Adjust the light intensity using the rocker switch.
Key to Fig. 5:
1
On / off switch (3, 3 materials stand), standby button (5, 5 materials stand and 7, 7 materials stand)
2
Sideport selector wheel
3
Condenser turret disk
4
Condenser centering screw
5
Polarizer D with 2-position filter changer
6
Condenser
7
Luminous-field diaphragm knurled wheel
8
Vertical adjustment knob for condenser
9
Slot for iris diaphragm slider and FL attenuator, discrete
10
Microscope stage (with inserted universal mounting frame K)
11
LM-Set button
12
Analyzer slider slot
13
Nosepiece
14
Compensator mount 6x20 (only for materials stands)
15
Reflector turret or dual filter wheel mot.
16
Optovar turret selector wheel
17
Coarse / fine focus drive
18
TL button for switching the LED / halogen illuminator or the reflected light shutter (fluorescence) on and off
19
Control wheel for LED light / halogen illuminator intensity control
20
Rotary / sliding button for vis / doc beam splitting
21
Rotary / sliding button for Bertrand lens and manual shutter
22
Binocular section of the tube
23
Eyepiece
24
Eyepiece focusing ring
18
HOW TO GET TO THE FIRST PICTURE
• Close the luminous-field diaphragm (Fig. 5/7)
until it is visible in the field of view (not
necessarily in focus) (Fig. 4/A).
• Bring the edge of the luminous-field diaphragm
into focus (Fig. 4/B) by adjusting the height of
the condenser (Fig. 5/8).
• Center (Fig. 4/C) the luminous-field diaphragm
using the centering screws (Fig. 5/4) and open
until the edge of the diaphragm just disappears
from the field of view (Fig. 4/D).
431004-6044-008
Axio Observer
/E).
Fig. 4
09/2017