Quantitation - Auxilab 4260/50 Manual De Usuario

Step 6. Measure samples
Put the sample to be measured in the main light path and put
Reference in the reference light path, then the result displays on
the screen automatically.

9. Quantitation

Step 1. Start Quantitation
Main menu, press
followed with
WL: 500.0nm
Main Menu
Basic Mode
①
Quantitative Mode
②
Wavelength Scan
③
Kinetics Mode
④
DNA/Protein Mode
⑤
Multi-Wavelength Mode
⑥
System Utility
⑦
Cancel
Step 2. Set Unit
Press to set concentration unit, press
EN TER
press to confirm.
Step 3. Establish Standard Curve or load the stored curve
Press to go into set up interface, 2 methods are under your
choice.
WL: 500.0nm
Quantitative Test
No. Abs
C=0.226+1.031*A+^1
Unit Fit Curve
Establish Standard Curve:
Method 1: Input Regression Equation
4) Set Fit Curve Method. Press
to choose the method and press
2
ABC
or press
EN TER
pressed to confirm.
10:00:00
D2
W
Move Up Move Dn Select
10:00:00
D2
W
Conc.(mg/ml)
WL.(nm)
500.0
Search
Scroll
r=0.962
, to choose "Quantitative Mode"
WL: 500.0nm
Quantitative Test
No. Abs Conc.(mg/ml)
C=0.226+1.031*A+^1 r=0.962
Unit Fit Curve
,
WL: 500.0nm
Calibration Table
No. Conc.(mg/ml)
1 0.000
C=0.226+1.031*A+^1 r=0.962
Method Params
to set Fit method，use
EN TER
to confirm.
10:00:00
D2
W
WL.(nm)
500.0
Search
Scroll
to choose and
10:00:00
D2
W
Abs
WL.(nm)
500.0
Standard Show Curve
,