Zeiss Axiolab 5 Manual Breve página 23

Axiolab 5
11 HOW TO GET TO THE FIRST PICTURE
Setting transmitted-light brightfield according to KÖHLER
− The microscope has been started up correctly.
− The microscope has been switched on.
• Adjust the image brightness using the light
intensity control ( F ig. 6/1) on the microscope
6 1 0 H
stand.
• Put a high-contrast sample into the specimen
holder of the mechanical stage.
• If condensers with a swiveling front lens are
used, swivel these into the beam path with
≥ 10x objectives and turn the knurled knob for
vertical adjustment of the condenser (
to the top stop. Make sure the stop is adjusted
to prevent the condenser from lifting out the
sample.
• On condensers with revolver/modulator disks:
turn the knurled ring (
B = brightfield.
• Bring the 10x objective into position on the
nosepiece ( F ig. 6/6) and focus the sample using
6 1 5 H
the focus drive ( F ig. 6/2).
6 1 6 H
• Close the field diaphragm (
make it visible in field of view (even if blurred)
(Fig. 6/A).
• Lower the condenser using the knurled knob
for vertical adjustment until the edge of the
field diaphragm appears sharp (
• Center the field diaphragm image with both
centering screws ( F ig. 6/4) on the condenser
6 2 0 H 6 1 7 H
carrier ( F ig. 6/C).
6 2 1 H 6 1 7 H
• Open the field diaphragm enough to make the edge of the diaphragm disappear from the field of
view ( F ig. 6/D).
6 2 0 H 6 1 7 H
• To adjust the aperture (contrast), take an eyepiece from the tube barrel. Looking through the barrel
adjust the aperture with the adjusting lever (
2/3 - 4/5 of the diameter of the objective exit pupil (
contrast at almost full resolution and is thus the best compromise for the human eye.
• Replace the eyepiece in the tube barrel.
07/2018
HOW TO GET TO THE FIRST PICTURE
F ig. 6/3)
6 1 1 H
F ig. 6/3) to position H (or
6 1 4 H
F ig. 6/5) enough to
6 1 7 H
F ig. 6/B).
6 1 9 H
F ig. 6/4) of the aperture diaphragm to the size of approx.
6 1 7 H
430037-7014-111
Fig. 6 Microscope adjustment in
transmitted light brightfield
microscopy
F ig. 6/E). In most cases this aperture gives the best
6 1 7 H
ZEISS
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