Section 4 - Performing Micro Volume Measurements; Pipetting Samples Onto The Micro Volume Read Head - Cole-Parmer JENWAY Genova Nano Manual De Instrucciones

SECTION 4 – PERFORMING MICRO VOLUME MEASUREMENTS
For detailed descriptions of the measurement modes that are available on the Genova Nano
spectrophotometer please refer to the supplied Genova Plus operating manual.
4.1

PIPETTING SAMPLES ONTO THE MICRO VOLUME READ HEAD

The Genova Nano spectrophotometer is designed to measure sample volumes ranging from
0.5µl to 5.0µl.
Jenway recommends that users should, if possible, use at least 2µl of sample for their
measurements.
Pipetting a sample onto the read head
Read head in rest position
Read head in measurement position
14
1. With the read head mechanism open,
pipette the liquid to be analysed onto the
centre of the lower read head. Ensure
there are no bubbles in the sample.
2. Close the lid of the spectrophotometer.
This will lower the read head assembly
down onto the path length drive motor. The
upper read head will now be in the rest
position, 2mm above the lower read head.
3. When a measurement is initiated, the path
length drive motor lowers the upper read
head to the specified
distance, the photometric measurement is
taken and the upper read head is then
returned to the rest position.
measurement