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19. The unit takes two readings at 260 and 330nm, at both 0.5mm and 0.2mm path lengths. At the
Press the button adjacent to the Circular Arrow icon to repeat the 2nd calibration solution
end of the readings, the unit will display the results of the measurements taken:
measurement or press the button adjacent to the Back Arrow icon to return to the 1st calibration
solution measurement screen.
15. The new calibration factor values must now be verified by re-measuring the air, blank and
standard values.
20. If the measured values are within the required tolerances, the Passes Test icon will be displayed.
Press the button adjacent to the Forwards Arrow icon, to continue, or press the button adjacent to
the Cross icon to abort the calibration process.
If the measured values are not within the required tolerances, the Fails Test icon is shown with an
error number. Refer to section 11 for a list of error codes.
Press the button adjacent to the Circular Arrow icon to repeat the blank solution measurement or
press the button adjacent to the Back Arrow icon to return to the air measurement screen.
21. The Calibration Solution Measurement icon will be displayed. Open the instrument lid and use a
lint free cloth to clean the read heads. Open the instrument lid and pipette a 2.0μl aliquot of the
calibration solution onto the lower read head. Close the instrument lid and press the key adjacent
to the Tick icon to initiate the calibration solution measurement.
22. The unit takes two readings at 260 and 330nm, at both 0.5mm and 0.2mm path lengths. The
variation from the expected Absorbance values are displayed.
20. If the measured values are within the required tolerances, the Passes Test icon will be displayed.
Press the button adjacent to the Forwards Arrow icon, to continue, or press the button adjacent
to the Cross icon to abort the calibration process.
23. If the measured values are within the required tolerances, the Passes Test icon will be displayed.
If the measured values are not within the required tolerances, the Fails Test icon is shown with an
Press the button adjacent to the Forwards Arrow icon, to continue, or press the button adjacent to
the Cross icon to abort the calibration process.
error number. Refer to section 11 for a list of error codes.
If the measured values are not within the required tolerances, the Fails Test icon is shown with an
error number. Refer to section 11 for a list of error codes.
Press the button adjacent to the Circular Arrow icon to repeat the blank solution measurement or
press the button adjacent to the Back Arrow icon to return to the air measurement screen.
Press the button adjacent to the Circular Arrow icon to repeat the calibration solution measurement
or press the button adjacent to the Back Arrow icon to return to the blank solution measurement
screen.
24. Following successful calibration, the instrument display will return to the micro volume accessory
settings screen and the new path length calibration factors will be shown.
24
16. The Air Measurement icon will be displayed. Open
the instrument lid and use a lint free cloth to clean
the read heads. Close the instrument lid and press
the key adjacent to the Tick icon to initiate the air
measurement.
17. The unit takes two dark and light readings at 260
and 330nm, at both 0.5mm and 0.2mm path lengths.
18. The Blank Solution Measurement icon will be
displayed. Open the instrument lid and pipette a
2.0μl aliquot of the matrix blank solution onto the
lower read head. Close the instrument lid and press
the key adjacent to the Tick icon to initiate the blank
measurement.
19. The unit takes two readings at 260 and 330nm, at
both 0.5mm and 0.2mm path lengths. At the end of
the readings, the unit will display the results of the
measurements taken:
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