MERCK Millipore Auto2D Guia De Inicio Rapido página 3

Load a Recipe
Choose SETTING > RECIPE. Touch the Recipe
Information screen. Recipe select dialog box
will appear.
Select "RECIPE NAME" > LOAD > OK > EXIT.
Note: Confirm the desired Recipe name is
displayed on upper right of the screen.
Chip Assembly
PAGE Chip
Cathode Side
E Chip
Anode Side
1. Remove the white tape on the
anode side of the Chip.
2. Carefully remove the plastic cover
on the cathode side of the chip.
3. Gently rinse anode and cathode buffer
wells with distilled water. Using a paper
cleaning wipe, carefully wipe any liquid
from the top of the Chip making sure not
to damage the thin strip of gel located
on the cathode side of the Chip.
20424817 Rev 06/21
All manuals and user guides at all-guides.com
Insert the Chips Into the Tray
Cathode Side
Anode Side
On the Auto2D Device touch screen, choose
®
OPEN > OK. Once tray is open, place the
PAGE Chip (light blue line) with the anode
side in front. Place the Solution Chip (dark
blue lines) on top of the PAGE Chip with the
cut-off corners in the front.
Apply the Solutions
1
3
3
2
1. Cathode Buffer, 4500 µL
2. Anode Buffer, 4000 µL
3. Distilled Water, 4000 µL
4. Working Equilibration Buffer
a. 700 µL
b. 700 µL (optional)
c. 700 µL (optional)
5. Working Rehydration Solution, 100 µL
6. Sample Solution, 10–12 µL
7. Molecular Weight Marker*
0.5–0.7 µL
* Cannot include SDS or
Bromophenol Blue
4
Place the Electrode Chip
1. IEF Electrode portion of Electrode Chip
and IEF Chip groove of Solution Chip.
2. Set the Electrode Chip onto the assembled
gel and solution chip so the  marking
faces front.
IEF Chip
7
6
5
a
b 4
c
Remove the IEF Chip protective film.
English 20424817 Rev 06/21
1
2
Insert the IEF Chip into the slot in the
Electrode Chip.
Before the Starting Eletrophoresis step, verify
the following:
• Electrode Chip is not wet except for anode
and cathode chamber.
• The clear cover on the cathode side of the
PAGE Chip is removed.
• Solution has been added where required.
Start Electrophoresis
Choose CLOSE > OK > START > OK.
Electrophoresis will begin.
5 English