The Use Of Down Illumination - Auxilab HBF001 Manual De Instrucciones

2. Wait for mercury lamp shining after starting, then close the light valve plate.
3. When the mercury lamp shines fully, open the light valve plate.
4. Place the fluorescent specimen (which is dyed by fluorescent pigment) on the stage,
then adjust focus to get image of fluorescent specimen by using the objective 4x or 10x
first.
5. As required depending on the fluorescent specimen pigment, choose the corresponding
fluorescent light filter group (rotating fluorescent group switch disc from 2 to 6) and
adjust fine focusing knob to get the image of fluorescent specimen in dark background
through eyepieces.
Note: please close the light valve when you don´t need to observe fluorescence; the
specimen should not be irradiated for long time by short wavelength light, otherwise,
the fluorescence will be faded.
Note: please don't turn on and shut off the mercury lamp in short time, the lamp must
be fully shined for a while after starting. After use and shut off the power supply, please
don't turn the lamp on again immediately, wait at least until the lamp is completely
cooled. When it is in use, open or close the mercury lamp light source by inserting and
removing the light valve.
6. Adjusting the field diaphragm
7. Adjusting the field diaphragm center adjusting screw makes the field diaphragm image
move to the field center. Pull the field diaphragm adjusting rod to make the field
diaphragm a little bigger than field of view.
8. Adjusting the aperture diaphragm
9. Take off one of the eyepieces and cover with a paper the top of the eyepiece tube; make
a small hole in the center and observe through the hole with the eye, the size of aperture
diaphragm (polygon) can be seen inside. Move the aperture diaphragm adjusting rod to
regulate it, the most appropriate size is about ¾ de diameter of the back focusing ground
of objective lens (circular hole). At this time, it does not diminish the resolution of
objective lens, but you can increase the contrast and make brightness of eyeshot
suitable for observation of eyes.
Notes
- When using, don´t touch the mercury lamp house to avoid burning skin
- In operation, the anti-uv shade plate (orange screen) must be installed, so as to avoid
damage in eyes.
- Interrupt the operation, close light valve plate immediately
- To insert and pull the power cable, it must be held by the plug, don´t pull the power
cable.
- Start observing once the mercury lamp is fully shined. If the power supply is shut off,
please don´t turn on the lamp again immediately; wait until it is completely cooled.
b) The use of down illumination
1. Close the diaphragm plate or shut off the mercury lamp power, turn the transmitted
light power switch on, and adjust the brightness through light intensity adjusting knob.
Manual de instrucciones- Instruction´s manual REF/CODE: HBF001
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