2. Set the fluorescent group switch disc at the neutral gear position "O".
3. Rotate the nosepiece and place 10X objective in the light path.
4. Place the samples on the stage.
5. Rotate XY coaxial hand wheel to move the sample into the light path.
6. Observe with the right eye through the right eyepiece and turn coarse focusing knob to
focus the sample; then adjust fine focusing knob to get a clear and sharp image.
7. Observe with the right eye through the left eyepiece and move the diopter adjusting
ring to make image clear in the left eyepiece. At this time, the image in both eyepieces
is in focus and clear
8. Regulate the eyepiece interpupillary distance.
9. Regulate filament center: remove one eyepiece and adjust filament focusing knobs and
filament upper/down, left/right adjusting knob until a clear filament image is observed
in the middle of the back focusing ground of objective lens
10. Observe with different magnification objectives and adjust the light intensity to the
required level. Since objectives are guaranteed the parafocal accuracy, to get a clear
image it is just necessary to adjust the fine focusing knob.
11. Insert the selected light filter into light path.
12. Regulate field diaphragm on the base until the field diaphragm is a little bigger than field
of view.
13. Regulate aperture diaphragm under condenser; take off one of the eyepieces and cover
with a paper the top of the eyepiece tube; make a small hole in the center and observe
through the hole with the eye, the size of aperture diaphragm (polygon) can be seen
inside. Move the aperture diaphragm adjusting rod to regulate it, the most appropriate
size is about ¾ diameter of the back focusing ground of objective lens (circular hole). At
this time, it does not diminish the resolution of objective lens, but you can increase the
contrast, and make brightness the eyeshot suitable for observation of eyes.
6. Safeguard and maintenance
o When cleaning all glass components, first blow the dust off the surface and then
gently clean with gauze; please wipe off oil or fingerprints carefully on the lens
surface with a few ether (70%) and alcohol (30%) mixed solution (dip the gauze or
absorbent cotton moistened with this organic solvent).
o Warning! As ether and alcohol are flammable liquids, they must be used with care;
pay attention not to make these chemicals close to the fire and EDM (electrical
discharge machining) source, such as switch operation in the electronic equipment.
Remember to use these chemicals only in a well-ventilated room.
o To clean other parts of microscopes use a soft hairless cloth; dip a small amount of
neutral cleaning agents and then wipe off the dirt.
o Please don´t break down any part of the microscope, which will damage the
microscope and lead to low function of performance.
Warning !
If the microscope is not operated as specified by this manual, it may be hazardous to the
safety of the users and may damage the equipment. Please always operate microscope in
accordance with this handbook.
Manual de instrucciones- Instruction´s manual
REF/CODE: HBF001
34