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Esquema de purificación de
oligonucleótidos
IEX HPLC
Separation of full length product from
shorter failure sequences based upon
size and differing charge to mass ration.
When trityl on there will be an on-
column cleavage of the trityl group
Desalting by gel-filtration
or ultra-filtration
Oligo ready to use
ÄKTA oligopilot plus Instrucciones de funcionamiento 28959748 AE
D Análisis y purificación de oligonucleótidos
Cleavage & Deprotectio n of oligonucleotide
from solid phase support
DMT off
DMT On
Reversed phase chromatography
Separation of more hydrophobic DMT containing
species from detritylated failure sequences
Removal of DMT group with acetic acid
Gel-filtration to remove small
molecules like salts and Trityl alcohol
Oligo ready to use
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