Optika Italy B-380 Manual De Instrucciones página 12

•
The goal is to superimpose this bright ring to the dark one, so that they are in the same position:
Image as seen in the telescope,
with centred rings.
•
Release the centering screws slowly, guiding them to the rest position.
•
Reinsert the eyepiece.
Now you can look at the phase contrast image of your sample.
The same procedure must be followed to align the other objectives (20x, 40x or 100x). Note that in order to ob-
tain a correct image with 100x, you have to use the oil immersion technique (oil between the objective and the
specimen coverslip).
Page 12