Using The Microscope; Adjust Interpupillary Distance - Optika Italy B-380 Serie Manual De Instrucciones

Using the microscope

Adjust the observation head
Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then lock the
lock-screw.
Place the specimen on the stage
Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is centred
over the stage opening by adjusting the coaxial knobs of the stage
LED lamp settings – Transmitted illumination
The microscope is fitted with high brightness white LED illuminator. Press the selector switch on the back of the
main body to I position. Turn the brightness adjustment knob to a brightness suitable for observation.
LED lamp settings – Epillumination
The microscope is fitted with high brightness LED illuminator. Press the selector switch on the back of the main
body to II position. Turn the brightness adjustment knob to a brightness suitable for observation.

Adjust interpupillary distance

Hold the right and left parts of the observation head by both hands and adjust the interpupillary distance by turn-
ing the two parts until one circle of light can be seen.
Focus and diopter adjustment
Turn the dioptric adjustment ring on the left eyepiece to the zero position. Turn the coarse focus knob in order
to focus the slide with an objective with low magnification. Adjust the fine focus knob until you obtain a clear and
defined picture observing with the right eye, and then act on the left dioptric compensation ring observing with
the left eye. When the image appears in focus, choose the necessary objective with the revolving nosepiece.
Turn the tension-adjust-knob to get a suitable tension for the focus system.
Condenser adjustment
Turn the condenser adjustment knob to move the condenser up or down for a clear and uniform sample illumina-
tion. The two condenser centring screws can be used to centre the condenser.
Set the numerical aperture
Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus controlling image
contrast and resolution.
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