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1. Technical introduction
1.1 Microfluidics technology: Microfluidics refers to the science and technology
involved in systems that microtubes (tens to hundreds of micrometers in size) to
process or manipulate tiny volumes of fluids (ranging from nanoliters to attoliters). It is
an emerging interdisciplinary discipline involving chemistry, fluid physics,
microelectronics, new materials, biology and biomedical engineering. Because of the
characteristics of miniaturization and integration, microfluidic devices are commonly
referred to as microfluidic chips, and also known as lab-on-a-chip devices and micro
total analysis systems.
1.2 Isothermal nucleic acid amplification technology: this is the general term for
a class of molecular biology technology that expands the copy number of a particular
DNA or RNA fragment at a particular temperature. At present, the main isothermal
amplification technologies include: rolling circle nucleic acid amplification,
loop-mediated isotemperature amplification, strand displacement amplification,
nucleic acid sequence-based amplification and helicase-dependent amplification. They
all share common characteristics: constant temperature, high efficiency, special, no
need for special equipment.
2. Main uses and scope of application
It can be used with adaptive microfluidic chips for in vitro amplification detection
of sample nucleic acid.
3. Device characteristics
■ The thermal circulation system adopts metal film heating technology, which has
stable and reliable performance.
■ The temperature changes rapidly, and the heating and cooling rate is no less than
10°C/min.
■ It is compatible with microfluidic chips independently developed by Guangzhou
Pluslife Biotech Co., Ltd.
Chapter I Overview
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