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Levenhuk 400 Series Microscopes
General Information
Read the user manual carefully before you start working with a microscope. Levenhuk 400 Series biological microscopes are
safe for health, life and property of the consumer and the environment when properly used, and meets the requirements of
international standards. These microscopes are designed for observing transparent objects in the transmitted light using the
bright field method. They perform well in clinical research and tests; teaching demonstrations; bacterioscopy and cytology
in medical and health establishments, laboratories, universities; and may be used for scientific research in agriculture and
microbiology.
Microscope assembly
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Unpack the microscope carefully and place it on a flat surface.
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Remove the plastic bags and dustproof cover of the eyepiece head.
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Install the monocular, binocular, trinocular head or LCD screen and tighten the locking screw.
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Inspect all the items included in the kit and define their purpose.
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Open the battery compartment, position the batteries according to the polarity marking on the battery compartment,
reinsert the battery compartment door and turn on the microscope.Before using the microscope, it is necessary to charge
the batteries: insert the batteries into the battery compartment (observe the polarity) and connect the microscope to the
mains using the power adapter.
Use
Please refer to the fig. 1а (monocular model), fig. 1b (binocular model), fig. 1c (trinocular model) and fig. 1d (model with
LCD screen).
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Turn on the power and the illumination will turn on. Set the brightness at approximately 70%. Place the specimen on the
stage. Make sure the clips hold the specimen firmly in place.
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Iris diaphragm should be adjusted in accordance with numerical aperture of a lens. The best practice is to make the iris
diaphragm slightly smaller than the aperture of the selected lens. Open or close the iris diaphragm using the adjustment
screws. If the adjustment screw is turned to the right, the iris diaphragm is fully open. Note: the diaphragm is not intended
for adjusting the brightness of the illumination. To adjust the brightness, use the brightness adjustment knob.
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Slide the filter holder, place the filter in it and then return the holder to its initial position.
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To change the objective magnification, turn the revolving nosepiece until it clicks.
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Make sure that the lens does not touch the sample when adjusting the focus: rotate the coarse focus knob until the
specimen is about 3.175mm (1/8") away from the lens.
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Looking into the eyepiece, slowly turn the coarse focusing knob until you can see the image of the observed specimen. Turn
the fine focusing knob to make the image clear. The fine focusing mechanism allows you to focus on the observed specimen
while using high magnifications.
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When you use an oil immersion objective, the space between the specimen and the lens should be filled with immersion oil.
Raise the condenser to the upper position and put a drop of oil on the lens and on the cover glass of the specimen. Move the
revolving nosepiece back and forth to get rid of air bubbles in the oil. Then, fix the objective lens in its working position.
Make sure that oil fills the entire space between the objective lens and the specimen. After use, wipe the lens dry.
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To adjust the position of the mechanical scale (except for Levenhuk 400M), turn the coarse focusing knob in a clockwise
direction if you want to fix the position of the mechanical scale, or in a counterclockwise direction if you want to loosen it.
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To place the specimen in the field of view, move the stage horizontally back and forth or left and right using the stage
moving knob (except for Levenhuk 400M).
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Look through the right eyepiece with your right eye and adjust the image sharpness with the coarse and fine focusing knobs.
Binocular and trinocular models: then, look through the left eyepiece with your left eye and rotate the diopter adjustment
ring to equalize the difference between your left and right eyes vision. Remember the setting for future use. Adjust the
distance between the eyepiece tubes of the binocular head so that the image merges into a single circle.
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Trinocular models: you can install the digital camera to the third eyepiece tube.
LCD screen (Levenhuk D407 LCD)
Getting started
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Connect the power cable to the power input on the LCD screen and connect it to the network. The red LED will light up.
Turn the LCD screen on with the power on/off button; the LED light will change to green.
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Insert the SD card (included) in the SD slot to view the image and save the resulting photos and videos.
Menu and functions
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Use the buttons on the back of the LCD screen to control the camera functions and parameters. After modifying the
parameters, exit the interface to save data.
Press the Menu button to call up the menu. Use ↑↓ to select the functions. Use → to enter the submenu. Press the Menu
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button once again to save the modified parameters and exit the interface.
Photo and video
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Photo. Press the Snap button on the LCD panel to take a photo. The image will be saved on the SD card.
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Video. Before recording a video, make sure that the SD card is formatted with the FAT32 file system and has enough free
space. You cannot take photos while recording a video. Enter the menu and select "Record Video" to record a video.
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