Agilent ZORBAX Serie Guia Do Usuário página 10

Storage recommendations
Long-term storage of silica-based, bonded phase columns
should be in a pure organic solvent such as acetonitrile. If
the column has previously been used with a buffered mobile
phase, the buffer should first be removed by purging the
column with 20 to 30 column volumes of a 50:50 mixture
of methanol or acetonitrile and water, followed by 20 to
30 column volumes of the pure solvent. Before storing,
end-fittings should be tightly capped with end-plugs to
prevent packing from drying out. To protect equipment, is it
desirable to remove salts from the instrument and column
by purging the column with the same mobile phase without
the buffer (for example using 60:40 ACN/H 0 to remove
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a 60:40 ACN/0.02 M phosphate buffered mobile phase).
Re-equilibration is rapid with the original mobile phase when
using this approach and any danger of corrosion from the
salts is eliminated.