Agilent ZORBAX Serie Guia Do Usuário página 9

Column care
The inlet frit on columns with a particle size of 2.7 µm or
greater has a nominal porosity of 2 µm. Samples that contain
particulate matter will plug the column inlet frit. ZORBAX
and Fast Guard columns and hardware kits (as required) are
recommended for use with such samples, and are generally
recommended for all column use.
See agilent.com/chem/fastguards for more information
about guard columns.
Cleaning your column/extending column life
For columns that can be backflushed (Poroshell 120,
ZORBAX columns with particles >1.8 µm and all Pursuit and
Polaris columns), start with a stronger (less polar) solvent.
1. Disconnect column from detector and run wash solvents
into a beaker.
2. Start with your mobile phase without buffer salts
(water/organic). Flush 10 to 20 column volumes through
the column.
3. Next, use 100% organic (methanol or acetonitrile).
4. Check pressure to see if it has returned to normal; if not,
then,
5. Discard column or consider stronger conditions, for
example, 75% acetontrile/25% isopropanol
6. Increase to 100% isopropanol, 100% methylene chloride
or 100% hexane (if you use methylene chloride or hexane,
you will need to flush the column with isopropanol prior to
use and before returning to your reversed-phase mobile
phase).
For columns with particles <1.8 µm, do not backflush the
column—replace the column.
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