Optika B-500TiFL Manual De Instrucciones página 10

4.0 USING THE MICROSCOPE
4.4 Always keep the iris diaphragm completely open when working with fluorescence light.
4.5 Brightfield use: Lamp settings
The microscope is fitted with a 3.5W X-LED
socket and turn on the switch on the back of the main body. Turn the brightness adjustment knob to a
brightness suitable for observation.
4.6 Adjust interpupillary distance
Hold the right and left parts of the observation head by both hands and adjust the interpupillary
distance by turning the two parts until one circle of light can be seen. After finishing the interpupillary
adjustment, turn the dioptric adjustment ring on the left eye tube to the "0" position at the graduation
line on the eyetube.
4.7 Focus and diopter adjustment
Loosen the focus-stop knob, rotate the coarse focusing knob to bring the slide into focus with an
objective with a low magnification, and then lock the focus-stop knob. Adjust the fine focusing knob to
get the image sharp and clear while observing with your right eye, then turn the left dioptric adjustment
ring to a sharp and clear image also with the other eye. Turn the tension-adjust-knob to get a suitable
tension for the focus system. When the image is in focus, turn the nosepiece to choose the objective
you need.
4.8 Brightfield use: Condenser adjustment
Turn the condenser adjustment knob to move the condenser up or down for a clear and uniform
sample illumination. The two condenser-centring screws can be used to centre the condenser.
4.9 Brightfield use: Set the numerical aperture
Adjust the aperture of the iris diaphragm located under the condenser, to set the numerical aperture
of the illuminator, thus controlling image contrast and resolution.
4.10 Brightfield use: Field diaphragm
Adjust the field diaphragm to align the system and to control stray light.
4.11 Brightfield use: Additional filters
The chromatic correction filter (blue) and the monochromator filter (green) can be inserted into the
filter holder for definition of specimen parts. The use of the green filter is recommended for phase
contrast observations.
4.12 Brightfield use: Using the polarizer (optional)
The polarizing set consists of two filters; the analyser and the polarizer. The analyser is placed in the
analyser holder (you have to remove the orange protection filter in order to access the holder) and
the polarizer on top of the illuminator.
4.13 Video capturing (optional)
B-500TiFL is provided with trinocular observation head. It can be connected to cameras via a photo/
video adaptor, for photo and video capturing. Before taking a picture or filming video, if necessary,
obscure with a dark cloth both the camera viewfinder and the eyepieces and pull out the light path
selector lever. Please refer to the adaptor and camera manuals for further details.
illuminator. Insert the plug of the cable into the power
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