Basic Colorimetry - HACH LANGE Pocket Colorimeter II Single Manual Del Usuario

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rCL Shows the last 10 measurements recorded. Push
recorded measurements (01—most recent measurement, 10—
oldest measurement). Push
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SCA Not applicable to the single wavelength models.
3. Push to go back to measurement mode.
Measurement

Basic colorimetry

Colorimetry measures the amount of color in a clear medium, such as
a liquid, to identify the quantity of a particular substance (the analyte) in
the liquid. Typically, the concentration of the analyte is proportional to
the intensity of the color in the clear medium (solution). In most
methods, a darker color indicates a higher analyte concentration.
Absorbance (Abs) at a specific wavelength is typically used to measure
the amount of light absorbed by the solution. Absorbance (Abs) is
calculated as:
Abs = –log T or Abs = –log (I
Where:
T = transmittance
I = intensity of the light transmitted through the sample
T
I = intensity of the light that enters the sample
O
Some substances, such as dyes and different metal ions, have
inherent color and can be measured without any additions. In most
cases, a chemical reaction between an indicator and the analyte is
necessary to get a colored product that can be measured.
Once the relationship between the amount of color (measured as
absorbance) and a known concentration of a sample is identified, the
instrument can be used to measure concentrations of unknown
samples. A user-entered calibration curve is used to measure the
sample concentration.
To identify the amount of color in a sample, the instrument measures
the amount of light the solution absorbs. The absorption of light is
dependent on the wavelength of the light and the color of the solution.
12 English
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