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9. Use of oil immersion objective (Fig.16)
1. Focus the specimen with a low power
objective.
2. Lower the stage (remembering to lock the
coarse upper limit knob).
► Put a drop of oil (provided) on the area
of the specimen to be observed. Make
sure that there are no oil bubbles. Air
bubbles in the oil damage the image
quality.
•
To check for bubbles: remove an eyepiece,
fully open the aperture diaphragm and
observe the objective exit pupil. (The pupil
must be round and bright).
•
To remove the bubbles, gently move the
nosepiece to the right and left to move the
immersion objective a few times and allow
the air bubbles to move.
3. Insert immersion objective.
4. Return the stage to the upper focusing
point and obtain an optimal focus using
the fine focus knob.
5. After use, gently remove the oil with a soft
paper towel or a lightly moistened optic
paper with a mixture of ethyl ether (70%)
and absolute ethyl alcohol (30%).
► The immersion oil, if not immediately
cleaned, could crystallize creating a
glass-like layer.
In this situation the observation of
the specimen would be difficult if not
impossible due to the presence of an
additional thickness on the objective.
Page 51
Fig.16
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