Using The Phase Contrast Set; Darkfield Condenser (Optional) - VWR isiScope 800 Serie Manual De Instrucciones

Using the phase contrast set

Phase contrast is provided as standard in TL824 PH model. The phase contrast set has 4 objectives for
phase contrast and one condenser with the dedicated rings of phase. To use the phase contrast, remove
the brightfield condenser from the microscope and replace it with the phase contrast condenser.
In order to obtain a correct phase contrast view, the phase contrast rings must be
aligned using the alignment screws placed on both sides of the condenser.
Follow these steps:
Select an objective by rotating the nosepiece (it's suggested to start with the 10X
objective), and focus on a slide under brightfield illumination ("BF" or "0" position
on the condenser wheel).
Insert the phase ring to be realigned (by rotating the wheel on the condenser until
you read the corresponding number, e.g. "10").
Remove an eyepiece, and replace it with the centring telescope.
Focus the centring telescope, without touching the microscope focus, in order to
focus the phase rings (one bright ring and one darker ring, as picture A).
Turning the two phase ring centring screws, move the bright ring, so that it is com-
pletely contained in the dark ring. (see picture B.)
Remove the telescope, and replace the eyepiece.

Darkfield condenser (optional)

For darkfield observation, the Abbe condenser in standard equipment must be replaced with a special con-
denser for darkfield, as showed in the picture.
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