Operation; Adjust The Observation Head; Place The Specimen On The Stage; Lamp Settings - VWR isiScope 800 Serie Manual De Instrucciones

Operation

Adjust the observation head

Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then lock the
lock-screw.

Place the specimen on the stage

Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is centred
over the stage opening by adjusting the X-Y translation knobs of the stage.

Lamp settings

The microscope is fitted with a 3,5W high-efficiency white led source. Insert the plug of the power supply into
the power socket and turn on the ON/OFF switch on the side of the main body. Turn the brightness adjust-
ment knob to a brightness suitable for observation.

Adjust interpupillary distance

Hold the right and left parts of the observation head with both hands and adjust the interpupillary distance by
turning the two parts until one circle of light can be seen. After finishing the interpupillary adjustment, turn the
dioptric adjustment ring on the left eyetube to the "0" position on the graduated line.

Focus and diopter adjustment

Loosen the focus-stop knob, rotate the coarse focusing knob to bring the slide into focus with an objective
with a low magnification. Adjust the fine focusing knob to get the image sharp and clear while observing with
your right eye, then turn the left dioptric adjustment ring to a sharp and clear image also with the other eye.
Turn the tension-adjust-knob to get a suitable tension for the focus system. When the image is in focus, turn
the nosepiece to choose the objective you need.

Condenser adjustment

Turn the condenser adjustment knob to move the condenser up or down for a clear and uniform sample il-
lumination. The two condenser centring screws can be used to centre the condenser.

Set the numerical aperture

Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus controlling im-
age contrast and resolution.

Field diaphragm

Adjust the field diaphragm to align the system and to control stray light.

Additional filters

The colored filter (green) can be inserted directly above the field diaphragm. The use of the green filter is
recommended for phase contrast observations.

Using the polarizer (optional)

The polarizing set consists of two filters: the analyzer and the polarizer. The analyzer is placed in the filter slot
under the head, while the polarizer directly above the field diaphragm.

Photo and Video capturing

The microscope has a dedicated trinocular head that allows to capture images via a photo/video adaptor,
specific for each model of camera. Before taking a picture or filming video, pull out the light path selector
lever (on the right of the optical head). Please refer to the adaptor and camera manuals for further details.
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