8.10 Use Of Fluorescence; Use Of The Polarizer (Optional) - Optika Italy B-290 Serie Manual De Instrucciones

8.10 Use of fluorescence
1. Operate on the main switch placed in the rear side of the
microscope.
• Setting on "I" turns on transmitted light, setting on "II" turns
on fluorescence. Setting on "O" turns off the microscope.
(Fig. 31)
2. Move the filter selector in the "B" position (Fig. 32) to insert
the fluorescence filter in the light path. Move the selector in
the middle to work with brightfield transmitted light.
• Unlike a mercury lamp system, B-290LD LED illumination
doesn't need any power-up time for heating, and can be used
immediately after switching on. Also, the LED source is pre-
aligned in factory and doesn't need any alignment operation.
3. Focus on your sample, and adjust the light intensity as need-
ed through the brightness adjustment knob. In order to im-
prove the darkness of the background (thus improving con-
trast), it is strongly suggested to put a dark cover on the light
exit at the base of the microscope.
FILTER EXCITATION
NAME FILTER
B 460/490 nm

8.11 Use of the polarizer (optional)

1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer until the
darkest position is achieved.
3. Once the dark is achieved ("extinction" or "Crossed Nicol"
position) it is possible to begin the observation.
DICHROIC MIR-
ROR
505 nm
Page 19
EMISSION
FILTER
• FITC: fluorescent antibodies
515LP nm • Achridine orange DNA/RNA
• Auramine
F ig. 31 F ig. 31
F ig. 32 F ig. 32
APPLICATIONS