9.2
Autoclaving
9.3
Reference run (rEF)
After each change of the pipette shaft, a manual reference run must be carried out. The reference
run is used for the secure coupling of the piston.
a. Call up rEF mode: Pressing the 'Menu' and 'Enter' keys simultaneously activates the rEF mode.
The display shows 'rEF'.
b. Carry out reference run: Pressing the pipetting key once triggers the reference run.
A clear functional noise can be heard.
After the reference run, the display automatically switches back to the previously set program.
10.
Maintenance
In order to ensure proper functioning, the ErgoOne® E Multi-Channel pipettes should be serviced
at regular intervals and cleaned as necessary.
What must be checked?
a. Check the pipette shafts, pistons, and seals for damage and contamination.
b. Check the device for leaks. Aspirate a sample and hold the instrument vertically for approx.
10 s. If drops form at the pipette tips, see Troubleshooting.
The highlighted part of ErgoOne® E Multi-Channel
pipettes can be autoclaved at 121°C (250 °F), 2 bar,
and with a holding time of at least 15 minutes according
to DIN EN 285.
a.
Eject the pipette tips.
b.
Separate the pipetting unit from the handle, see
Maintenance.
c.
Autoclave the complete pipetting unit without
further disassembly.
d.
Allow the pipetting unit to cool down completely
and dry.
e.
Screw the pipetting unit back into the handle.
f.
Carry out reference run (rEF).
NOTICE!
The effectiveness of autoclaving must be verified by
the user. Maximum safety is achieved through vacuum
sterilization. We recommend the use of sterilization bags.
If the pipette is autoclaved frequently, the piston and
seal should be greased with the grease supplied in
order to ensure proper movement.
ErgoOne
E Multi-Channel Pipettes · User Manual · 25
®