10.10 Use of the aperture diaphragm
•
The Numerical Aperture (N.A.) value of the aperture
diaphragm affects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of
focus of the image.
•
With low contrast specimens pull the lever ③ "AS" (Fig.
27) to slightly close the aperture diaphragm. If necessary,
remove on eyepiece and, looking into empty sleeve, adjust
the diaphragm's lever in order to obtain an image like the one
in Fig. 29.
10.11 Light path selection
Pull the light path selector lever ① to the left or to the right using
your thumb, selecting the light path you need. (Fig. 30)
POSITION
In
Out
10.12 Use of color filters
1. Grab the filter holder handle ② and move the slider to the
left or to the right in order to insert the desired one. (Fig. 31)
FILTER
Converts the color temperature of the LED
Blue
into daylight
Increases the contrast in black and white pho-
Green
tography
Reduces lighting irregularities by creating a
Frosted
homogeneous illumination
10.13 Use of polarized light
1. Remove the specimen from the stage.
2. Put a mirror on the stage and get into approximate focus on
the mirror surface.
3. Insert polarizer and analyzer into the light path.
4. Rotate the knurled ring of the analyzer ① and, looking into
the eyepiece, reach the darkest position available. (Fig. 32)
5. Once the dark is achieved ("extinction" or "Crossed Nicol"
position) it is possible to begin the observation.
6. Remove the mirror from the stage, put a specimen and focus.
LIGHT
50% EYEPIECES - 50% TV
100% EYEPIECES
USE
Page 16
APERTURE
DIAPHRAGM
FIELD OF VIEW
①
70-80%
30-20%
F ig. 29
F
ig. 29
①
F ig. 30
F
ig. 30
②
F ig. 31
F
ig. 31
F ig. 32
F
ig. 32