OPTIKA MICROSCOPES B-500DK Manual De Instrucciones página 13

4.0 B-500DK DARKFIELD MICROSCOPE CONFIGURATION
• Swing the provided special 100X immersion objective to a position in the nosepiece where it
will be the next objective rotated into the optical pathway directly above the specimen.
• Position the area to be imaged in the center of the microscope's optical path using lower
power objective (e.g. 10x or 40x). Next, the oil immersion objective (without oil) is placed
into the optical path and the specimen area of interest is roughly brought into center of the
viewfield and focused. This pre-positions all of the components of the system in preparation
for the addition of oil. Swing the immersion objective to an adjacent stop on the microscope
nosepiece and apply oil both to the objective front lens and specimen as described in the next
point.
• Use a piece of lint-free paper or an eyedropper to place a small droplet of oil directly on the
front lens of the immersion objective. Next, place another droplet of oil on the cover glass
immediately above the area to be imaged.
• Next, quickly rotate the oiled objective into position above the specimen merging the two oil
droplets (one on the sample and one on the objective) into a single pool. A bright flash should
occur (from scattered light) the instant oil on the front lens of the objective comes into contact
with the microscope slide.
• Check for air bubbles by observing the back focal plane of the objective with the provided
centering telescope. If bubbles are present, swing the objective to the next detent stop on the
microscope nosepiece and wipe off excess oil with a piece of lint-free lens tissue. Re-apply
oil to the objective front lens and try again.
• With the centering telescope, and the 100x objective inserted, check that the condenser is
well centered (a symmetrical ring of light must be seen). Finely center using the condenser
centering screws.
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